Co-existence of bla NDM-5 and tet(X4) in international high-risk E. coli clone ST648 of Human origin in China
Research Article: Muhammad Shafiq, Mi Zeng, Budi Permana, Hazrat Bilal, John Anderson, Fen Yao, Abdelazeem M. Algammal, Xin Li, Yumeng Yuan1 and Xiaoyang Jiao1.
Published in Frontiers in Microbiology, 2022
Abstract
The emergence of pathogens conferring resistance to last-resort therapies like tigecycline, colistin, and carbapenems limiting the therapeutic options and raising concerns about the emergence of new “superbugs”. The current study reports the first incident of a blaNDM-5 and tet(X4) co-harboring E. coli with resistance to carbapenem and tigecycline recovered as causative agent of a urinary tract infection in a 94-year-old patient. The E. coli strain ECCL209 carries multiple resistance genes (i.e., blaTEM-1B, blaNDM-5, blaCMY-2, aadA22, florR, erm(B), mph(A), erm(42), lnuG, qnrS1, and sul2), and exhibits resistance to almost all clinically used antibiotics. MLST analysis found that the strain belongs to ST648, considered as a worldwide high-risk pandemic clone. Moreover, multiple plasmid incompatibility types were detected, i.e., IncHI1A, IncHI1B, IncFII, IncFIA, IncFIB, IncQ1, Col, IncX4. Genetic analysis revealed that blaNDM-5 and tet(X4) genes were localized on two hybrid plasmids with multiple replicons. Continuous monitoring studies are suggested to quantify the antimicrobial resistance and assess the dissemination of such superbugs into a human health care setting.
The paper is available here
Recommended citation: Shafiq M, Zeng M, Permana B, Bilal H, Huang J, Yao F, Algammal AM, Li X, Yuan Y, Jiao X. Coexistence of blaNDM-5 and tet(X4) in international high-risk Escherichia coli clone ST648 of human origin in China. Front Microbiol. 2022 Nov 10;13:1031688. doi: 10.3389/fmicb.2022.1031688. PMID: 36439817; PMCID: PMC9685555.